Finding reliable antibodies that respond to new scenarios usually takes a bit of trial and error, but there are steps you can take to ensure this transition period is as smooth and quick as possible.
The following tips serve as a guide for selecting antibodies for new target proteins in your lab, but can also be used as a general guide for purchasing antibodies.
1. Some places to start looking for antibodies are antibody comparison sites (useful ones include Antibody, Labome, and Antibody Resources) and search engines (Google Scholar has an edge). The custom rabbit monoclonal anitbody is also one of the best antibodies for your research requirement.
Image Source: Google
Comparison sites let you weigh cost and application data from multiple providers in one place. Also, look up the names of your target genes and proteins to get as many results as possible.
2. Search literature; some publications already contain antibodies targeting the protein you are interested in? If so, make sure you include it in your comparison by placing it at the top of your list.
Use your judgment here – is the publication using antibodies in the application you want to use? Does the data meet your standards? If the answer to any of these questions is no, consider your alternatives.
3. Your application and the target chosen are usually good indicators of whether you need polyclonal or monoclonal antibodies. You will also determine what type of immunogen you want your antibodies to fight against. If you are clear about these points from the start, you can narrow your search easily.